How to Grow Mushrooms from a Spore Print

by | Feb 12, 2025 | Uncategorized

Growing mushrooms from a spore print is a rewarding process that allows you to cultivate a wide variety of fungi from scratch. Whether you’re interested in gourmet mushrooms like oyster or shiitake, or you’re simply exploring mycology as a hobby, this guide will walk you through the essential steps. Before we begin, it’s important to note that the cultivation of Psilocybe cubensis and other psychoactive species is illegal in the UK. This guide is strictly for legal mushroom species suitable for home cultivation.

What is a Spore Print?

A spore print is a collection of microscopic spores left behind when a mature mushroom cap is placed on a piece of paper or foil. This print contains all the genetic material needed to grow new mushrooms, making it a great way to propagate fungi.

What You’ll Need

To grow mushrooms from a spore print, you’ll need:

  • A clean, sterile workspace (consider looking at the link below on how to make a still air box)
  • Spore print of a legal mushroom species
  • Sterile water
  • Sterile Syringe (to create a spore syringe)
  • Agar plates or sterilized grain (for inoculation)
  • Pressure cooker or sterilization method
  • Growing substrate (such as sawdust, straw, or grain, depending on species)
  • Fruiting chamber

Read more: How to make agar plates

Read more: How to make a still air box

Step 1: Creating a Spore Syringe

A spore syringe is an easy way to introduce spores into your growing medium. Follow these steps:

  1. Sterilize Everything – Ensure your work area, syringe, and tools are thoroughly sterilized to prevent contamination.
  2. Scrape Spores into Sterile Water – Use a sterilized scalpel or knife to scrape a small amount of spores from the spore print into a glass of sterile water.
  3. Draw the Solution into a Syringe – Suck the spore-infused water into a sterile syringe. This solution can now be used to inoculate your growing medium.

Read more: How to make a spore syringe

Step 2: Inoculating Your Growing Medium

Depending on the type of mushroom you’re cultivating, you’ll need to inoculate either an agar plate or a sterilized grain jar.

  • For agar plates: Inject a few drops of the spore solution onto a sterilized agar plate. This helps isolate strong mycelium growth before transferring it to grain.
  • For grain jars: Inject about 1-2ml of the spore syringe solution into the grain jar through an inoculation port or self-healing injection site.

Keep your inoculated medium in a warm, dark area (around 22-25°C) and wait for mycelium growth to begin.

Read more: How to make agar plates

Step 3: Transferring to Bulk Substrate

Once your grain is fully colonized with mycelium, it’s time to transfer it to a bulk substrate suitable for your mushroom species. Some popular options include:

  • Oyster Mushrooms – Straw or coffee grounds
  • Shiitake Mushrooms – Hardwood sawdust blocks

Mix your colonized grain with the bulk substrate in a clean environment, then place it into a fruiting chamber with proper humidity and airflow.

Step 4: Fruiting and Harvesting

After a few weeks, you’ll see mushroom pins forming. Increase humidity to around 85-95% and maintain fresh air exchange. Within days, your mushrooms will mature and be ready for harvest!

Final Thoughts

Growing mushrooms from a spore print is a fascinating and fulfilling process. With proper sterilization, patience, and the right conditions, you can enjoy fresh, homegrown mushrooms with ease.

For high-quality spore prints, spore syringes, and spore swabs, check out ShroomSpores.co.uk. 

Disclaimer: This guide is for informational purposes only. Always research your local laws before attempting mushroom cultivation.

0
    0
    Your Cart
    Your cart is emptyReturn to Shop